MOLECULAR PLANT PATHOLOGY AND BIOCONTROL AGENT DESIGN

Abstract

The paper is an entire experimental overview of using molecular plant pathology in conjunction with the development of biocontrol agents to handle plant diseases in an environmentally beneficial manner.  We cultured and enumerated the pathogens in-vitro, screened bacterial and fungal antagonists in-vitro and in-vivo.  We applied a quantitative PCR (qPCR) and the estimation of the size of lesions to observe the effectiveness of suppressing the phenomenon of pathogens. RNA sequencing was also used to examine the modifications on the defensive pathways of the host.  Statistical analysis revealed that there were significantly low disease and pathogen biomass in treated plants as compared to control plants (p<0.05).  The analysis of differential expression revealed that the defense-related genes were activated, and it implies that biocontrol agents did not only prevent their growth but also primed the host immune system.  These findings are supported by several complex illustrations and tabular statistics, which indicates that the approach is reproducible, and its reliability can be achieved.  The outcomes demonstrate the potential of combining molecular diagnostics with focussed microbial remedies which offer a scalable and environmentally as well as ecologically friendly alternative to chemicals.   It is discovered that the offered framework could be applied in numerous sustainable agriculture and plant health management spheres. Also, it might serve as an example of further studies of the interaction of plants, microbe, and pathogen.